Topiramate Promotes Proliferation of Neural Stem Cells in Vitro

OBJECTIVE:To study the effects of topirmate on proliferation of neural stem cells in vitro.METHODS:Primary neural stem cells from newborn mice were cultured.The neural stem cells were incubated together with topiramate at 0.4,2 or 10 μmol·L-1.A vehicle control and positive control were set up,respectively.The quantity and viability of neural stem cells newly generated were analyzed by a convert phase microscope,the neurosphere numbers were counted,and neural stem cells viability was determined and a MTT assay.RESULTS:The number of neurospheres of groups treated with topiramate at 2 and 10 μmol·L-1 are 20.67±4.04,49.68±3.79,respectively,and the value of MTT assay of the two groups are 0.354 6±0.070 5,0.501 8±0.036 9,it was higher than the vehicle control neural stem cells(7.34±2.31、0.288 0±0.009 3,P0.01).CONCLUSION:Topiramate has the potential for the stimulation of proliferation of neural stem cells.