Trigonelline is a bitterness alkaloid isolated from fenugreek Papilionoideae legume genus dried fenugreek seeds. In Chinese and Western medicine pharmacopoeia, trigonelline has amounts of applications. Trigonelline could also be extracted from a cafee beans, and it is synthesis of a lot of important aromatic substances. Drinking coffee may reduce the risk of cancer and some chronic diseases. At present there are amounts of methods for the determination of coffee trigonelline, each of which has pros and cons. Describe five methods for the determination of coffee trigonelline: spectrophotometric method, ion chromatography, paper chromatography, high performance liquid chromatography and liquid chromatography-mass spectrometry. In the end, it is summarized that the latest developments in the various detection methods of coffee trigonelline content.