Objective To acquire the brain fatigue symptoms reproducibility of tongue manifestation used in the diagnosis of brain fatigue. Methods Application of TLAG type tongue instrument with expert knowledge system and computer tongue features were extracted from the analysis and tongue microcirculation,observation of surface structure of tongue according to the diagnosed brain fatigue in 874 patients with systematic observation and analysis of tongue picture. Results Brain fatigue group tongue less spirit and non-spirit was 100%,pathological change in papillae of tongue was 100%,tongue texture change was 75. 74% and tongue shape change was 58. 55%. With the observation of tongue tip microcirculation,abnormal capillary form rate was 70%,blood stasis rate was 66. 66%. With the surface structure observation of tongue,abnormal papillae of tongue was 100%,widening physiological pattern was 63. 33%. The tongue color change in reference group was 100%,the tongue coating change was 71%,the tongue shape change was 68% and the tongue spirit change was 39%. Conclusion Brain fatigue change of tongue picture has a unique variation law: The tongue spirit and tongue papillae are the first,the tongue picture and tongue shape are the second.Compared with the reference group,the differences are obvious. P 0. 01.