CCR2型
脊髓
神经病理性疼痛
小胶质细胞
骨癌
趋化因子受体
医学
免疫印迹
下调和上调
免疫组织化学
趋化因子
受体
神经科学
病理
生物
内科学
癌症
麻醉
炎症
基因
生物化学
作者
Jihua Hu,Mengyao Wu,Min Tao,Jianping Yang
标识
DOI:10.1016/j.brainres.2013.03.002
摘要
Accumulating evidence suggests that chemokine CC motif receptor 2 (CCR2) plays an important role in neuropathic pain. It has been shown that spinal CCR2 is upregulated in several neuropathic pain models and expressed by neuronal and glial cells in the spinal cord. In this study, we investigated the expression changes and cellular localization of spinal CCR2 in a rat model of bone cancer induced by Walker 256 cell inoculation. The present results indicated that mechanical allodynia progressively increased in bone cancer pain (BCP) rats. Western blot and immunohistochemical analysis demonstrated that the expression of CCR2 in the spinal cord was significantly increased on day 6, 12, and 18 in BCP rats, with a peak on day 6. Furthermore, double immunofluorescence labeling indicated that CCR2 was expressed by both microglia and neurons in the spinal cord. These results suggest that CCR2 may be involved in the development of BCP, and that targeting CCR2 may be a new strategy for the treatment of BCP.
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