Development of a bi-modality XCT: fDOT instrument

We present first results of a fluorescence optical diffusion tomography experiment coupled to a X-ray computed tomography reconstruction. An instrument, dedicated to the co-registration of optical and X-ray measurements, has been developed: specific acquisition protocol and reconstruction software have been developed for carrying out fluorescence diffuse optical tomography in a cylindrical geometry consistent with X-ray tomography. Actual animal geometry provided by the X-ray tomography is used to give animal boundaries to the diffuse optical tomography reconstruction algorithm. Experiments have been conducted on sacrificed mice and fluorescence reconstructions have been evaluated and are geometrically consistent with X-ray ones.