化学
电化学发光
检出限
冠状病毒
猪流行性腹泻病毒
过氧二硫酸盐
纳米材料
纳米技术
色谱法
2019年冠状病毒病(COVID-19)
钾
生物化学
材料科学
有机化学
传染病(医学专业)
病理
基因
疾病
医学
作者
Jing Ma,Wenjing Wang,Yun Li,Zhicheng Lü,Xuecai Tan,Heyou Han
标识
DOI:10.1021/acs.analchem.0c03836
摘要
The sensitive detection of coronavirus is of vital importance for the prevention of its rapid spread. Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea and death in neonatal piglets. In this work, a novel PCN-224-based electrochemiluminescence (ECL) system was constructed for PEDV detection with high sensitivity. We found that PCN-224 can be employed as an ECL reporter with a strong signal because of its zirconium-based organic porous frame nanomaterial with a large specific surface area and stable structure. TiO2 nanoparticles were used as an accelerator for the first time to promote the reduction of coreactant potassium peroxydisulfate on the cathode; thus, the initial ECL signal of PCN-224 was significantly amplified. In the presence of PEDV, the ECL signal decreased due to the block effect to electron transfer. As a result, the novel “signal off” biosensor achieved a sensitive detection of PEDV ranging from 1 pg/mL to 10 ng/mL, with a detection limit of 0.4 pg/mL (S/N = 3). Importantly, the PCN-224 nanomaterial enriched the ECL system in biological analysis, and the proposed strategy provided a new route for coronavirus detection.
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