化学
组胺
纳米棒
拉曼光谱
线性范围
表面增强拉曼光谱
色谱法
组合化学
纳米技术
核化学
检出限
拉曼散射
医学
光学
物理
内科学
材料科学
作者
Ting Zhou,Min Fan,Ruiyun You,Yue Lu,Luqiang Huang,Xu Yu,Shouhua Feng,Yang Wu,Huiying Shen,Lanjin Zhu
标识
DOI:10.1016/j.aca.2020.01.017
摘要
We have successfully prepared a highly sensitive sandwich nanosensor combined Fe3O4 and [email protected]@Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe3O4 beads with –COOH served as a capture part to enrich histamine. The [email protected]@Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)-l-lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10−3-10−8mol/L showed a linear trend with R2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection.
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