基因沉默
肌腱
脚手架
粘附
细胞生物学
RNA干扰
基因表达
生物医学工程
再生(生物学)
小RNA
化学
生物
解剖
基因
医学
核糖核酸
生物化学
有机化学
作者
Guangjie Wu,Binbin Sun,Chunfeng Zhao,Zhuoying Wang,Songsong Teng,Mengkai Yang,Zhi Cui,Guoliang Zhu,Yinxian Yu
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2021-11-01
卷期号:7 (12): 5739-5748
被引量:13
标识
DOI:10.1021/acsbiomaterials.1c00747
摘要
Tendon adhesion formation is associated with the aberrant expression of many genes, and interfering with the expression of these genes can prevent adhesion and promote tendon repair. Recent studies have found that silencing the transforming growth factor β-1 (TGF-β1) gene can reduce the occurrence of tendon adhesions. The development of tissue engineering and three-dimensional (3D) printing technology have provided new solutions for tendon repair. In this study, TGF-β1 gene silencing microRNA (miRNA) based RNAi plasmid was loaded on a 3D tendon scaffold using 3D printing technology. In vitro experiments confirmed the sustained release of plasmid and the good biocompatibility of the printed tendon scaffold. Subsequently, the TGF-β1 gene silencing plasmid loaded tendon scaffold was implanted in a chicken tendon defect model to evaluate the effect of the scaffold in vivo. The results from biomechanical tests and histological examinations showed that the scaffold not only promoted tendon regeneration but also prevented tendon adhesion, which was conducive to the recovery of biofunction. Evaluation of protein expression showed that the loaded plasmids prevented tendon adhesion and promoted tendon functional repair via silencing of the TGF-β1 gene.
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