化学
光动力疗法
光敏剂
荧光寿命成像显微镜
荧光
生物物理学
对偶(语法数字)
生物
光学
光化学
物理
文学类
艺术
有机化学
作者
Yizhong Shen,Qian Tian,Yidan Sun,Jing‐Juan Xu,Deju Ye,Hong‐Yuan Chen
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2017-12-07
卷期号:89 (24): 13610-13617
被引量:83
标识
DOI:10.1021/acs.analchem.7b04197
摘要
Targeted delivery of intracellular stimuli-activatable photosensitizers (PSs) into tumor cells to achieve selective imaging and on-demand photodynamic therapy (PDT) of tumors has provided a vital opportunity for precise cancer diagnosis and therapy. In this paper, we report a tumor targeting and adenosine triphosphate (ATP)-activatable nanophotosensitizer Apt-HyNP/BHQ2 by modifying hybrid micellar nanoparticles with both nucleolin-targeting aptamer AS1411 and quencher BHQ2-labeled ATP-binding aptamer BHQ2-ATP-apt. We demonstrated that both of the fluorescence emissions at 555 and 627 nm were quenched by BHQ2 in Apt-HyNP/BHQ2, resulting in low PDT capacity. After selective entry into tumor cells through nucleolin-mediated endocytosis, the high concentration of intracellular ATP could bind to BHQ2-ATP-apt and trigger Apt-HyNP/BHQ2 dissociation, leading to turning “on” both fluorescence and PDT. The “off–on” fluorescence emissions at both 555 and 627 nm were successfully applied for dual color fluorescence imaging of endogenous ATP levels and real-time monitoring of intracellular activation of Apt-HyNP/BHQ2 in tumor cells. Moreover, imaging-guided precise PDT of tumors in living mice was also demonstrated, allowing for selective ablation of tumors without obvious side effects. This study highlights the potential of using a combination of tumor-targeting and ATP-binding aptamers to design ATP-activatable PSs for both fluorescence imaging and imaging-guided PDT of tumors in vivo.
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