红景天苷
酪醇
葡萄糖基转移酶
化学
生物合成
生物化学
酚类
酶
色谱法
作者
Guosi Li,Qilin Xu,Nan Hu,Xinyang Liu,Yiqi Jiang,Hailong Xue,Yongjun Zang,Furong Zhu
出处
期刊:Research Square - Research Square
日期:2023-02-28
标识
DOI:10.21203/rs.3.rs-2513556/v1
摘要
Abstract Objective Salidroside is an important plant-derived aromatic compound with diverse biological properties. The main objective of this study was to synthesize salidroside from tyrosol using UDP-glucosyltransferase (UGT) with in situ regeneration of UDP-glucose (UDPG). Results The UDP-glucosyltransferase 85A1 (UGT85A1) from Arabidopsis thaliana ,which showed high activity and regioselectivity towards tyrosol, was selected for the production of salidroside. Then, an in vitro cascade reaction for in situ regeneration of UDPG was constructed by coupling UGT85A1 to sucrose synthase from Glycine max ( Gm SuSy). The optimal UGT85A1- Gm SuSy activity ratio of 1:2 was determined to balance the efficiency of salidroside production and UDP-glucose regeneration. Different cascade reaction conditions for salidroside production were also determined. Under the optimized condition, salidroside was produced at a titer of 6.0 g/L with a corresponding molar conversion of 99.6% and a specific productivity of 199.1 mg/L/h in a continuous feeding reactor. Conclusion This is the highest salidroside titer ever reported so far using biocatalytic approach.
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