Solid-phase Extraction and High Performance Liquid Chromatographic Determination of Folic Acid in Fortified Foodstuffs: a Recommended Process Utilizing a New Strong Anion Exchange Sorbent

A highly efficient solid-phase extraction (SPE) column filled with a silica-based strong anion exchange sorbent was reported in the current study. The fabricated cartridge was used for the extraction and pre-concentration of folic acid in different standard and real samples prior to their chromatographic (HPLC-UV) investigation. Wide linear range (0.02‒1.2 µg/mL), low detection limit (0.006 µg/mL), low quantification limit (0.02 µg/mL), good relative standard deviation (2.6%), and good relative recoveries (96.0%) were attained under the optimum conditions. This cartridge was fabricated with accessible and inexpensive materials and can replace expensive commercial columns. In addition, simple synthesis steps, long service lifetime, and great extraction capability were some of the advantages of the proposed SPE column. The proposed technique was not affected by the interferences of sample matrices and can be successfully used for the routine determination of weak acidic substances including folic acid (at low levels).