低温保护剂
低温保存
球体
过冷
条件作用
冰核
细胞生物学
成核
细胞存活
化学
生物物理学
细胞外
脯氨酸
生物
生物化学
细胞凋亡
热力学
体外
氨基酸
物理
有机化学
统计
胚胎
数学
作者
Yanan Gao,Akalabya Bissoyi,Nina L. H. Kinney,Thomas F. Whale,Qiongyu Guo,Matthew I. Gibson
摘要
Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.
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