Architecting Ultrathin Graphitic C3N4 Nanosheets Incorporated PVA/Gelatin Bionanocomposite for Potential Biomedical Application: Effect on Drug Delivery, Release Kinetics, and Antibacterial Activity

Planar (2D) nanomaterials are garnering broad recognition in diverse scientific areas because of their intrinsic features. Herein, bulk graphitic carbon nitride (g-C₃N₄) was prepared from melamine, which was exfoliated to produce g-C₃N₄ nanosheets. The prepared g-C₃N₄ nanosheets were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), photo luminescence (PL) spectroscopy, and dynamic light scattering (DLS). The stable dispersion of a g-C₃N₄ nanosheet was incorporated into a PVA/Gelatin matrix to explore its efficacy as a promising drug carrier. A remarkable 42% increase in tensile strength for 1% g-C₃N₄/PVA/Gelatin was attained compared with that of the PVA/Gelatin film. Thermal stability increased due to addition of g-C₃N₄ nanosheet in the PVA/Gelatin film, where the maximum thermal degradation temperature increased by 9.5 °C when the 1% nanosheet was added to the PVA/Gelatin film. Moreover, the g-C₃N₄ nanosheets and g-C₃N₄/PVA/Gelatin showed no cytotoxicity against HeLa and BHK-21 cells. To investigate the in vitro drug releasing efficacy, ciprofloxacin was incorporated into g-C₃N₄/PVA/Gelatin. Experimental results showed a 62% drug release within 120 min at physiological pH 7.4. The data was curve fitted by different kinetic models of drug release to understand the drug release mechanism. The experimental data was found to fit best with the Higuchi model and revealed the diffusion control mechanism of drug release. Additionally, antibacterial study confirmed the drug release potency from g-C₃N₄/PVA/Gelatin film on both Gram-positive and Gram-negative bacteria. The above-mentioned promising findings might lead to an opportunity of using g-C₃N₄ as a potential drug carrier.