化学
甲苯胺酮
检出限
免疫分析
电化学发光
席夫碱
甲胎蛋白
线性范围
电化学
核化学
重复性
色谱法
基质(水族馆)
胶体金
纳米颗粒
高分子化学
纳米技术
肝细胞癌
有机化学
电极
抗体
材料科学
物理化学
癌症研究
生物
免疫学
海洋学
地质学
作者
He-Nan Sun,Miao Wang,Hongsheng Tan,Huiping Liu,Mingjun Liu,Shan-Shan Li
出处
期刊:Talanta
[Elsevier]
日期:2024-06-01
卷期号:273: 125876-125876
标识
DOI:10.1016/j.talanta.2024.125876
摘要
The high level of alpha-fetoprotein (AFP) expression is closely related to hepatocellular carcinoma (HCC). Herein, a dual signal ratiometric electrochemical immunosensor based on chitosan-ferrocenecarboxaldehyde-spindle gold (Chit-Fc-SAu) and Co/Fe metal-organic framework-toluidine blue/polydopamine (Co/Fe MOF-TB/PDA) was proposed for quantitative analysis of AFP. Specifically, Chit-Fc-SAu worked as a substrate to trap more primary antibodies (Ab1) generating the first electrochemical signal from Fc. Thanks to the large specific surface area, the synergistic and electronic effects of Co/Fe MOF nanosheets, and the rich functional groups of PDA, Co/Fe MOF-TB/PDA could load more secondary antibodies (Ab2) and signal molecules (TB) providing another amplified electrochemical signal. In the presence of AFP, Ab1-AFP-Ab2 formed a sandwich structure, and as the AFP concentration increased, the peak current ratio of TB to Fc (ITB/IFc) also increased. The dual signal ratiometric strategy can avoid environmental signal interference and achieve signal self-calibration, thereby improving the accuracy and reproducibility of detection. After a series of exploration, this self-calibrated ratiometric immunosensor exhibited a wide linear range (0.001-200 ng mL-1), a low detection limit (0.34 pg mL-1), and good repeatability. When applied to the assay of clinical serum samples, the detection results of ratiometric sensor were consistent with that of commercial electrochemiluminescence (ECL) immunoassay, significantly superior to that of non-ratiometric sensor. The self-calibrated strategy based on ratiometric sensor helps to improve the accuracy of AFP in clinical diagnosis.
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