化学
抗氧化剂
类胡萝卜素
超氧化物歧化酶
生物化学
活性氧
阿布茨
DPPH
一氧化氮
谷胱甘肽
丙二醛
药理学
肽
生物
抗菌肽
酶
有机化学
作者
Weichen Xiong,Jianpeng Xie,Yan Liang,Jinwei Chai,Ruiyin Guo,Baishuang Zeng,Jiena Wu,Shian Lai,Haiyun Zhang,Xiaowen Huang,Xin Chen,Xueqing Xu
标识
DOI:10.1016/j.ejphar.2023.175941
摘要
Chansu, a mixture extracted from Duttaphrynus melanostictus or Bufo gargarizans Cantor, is a traditional Chinese medicine with a broad range of medical applications. However, the peptides/proteins in it have not received adequate attention. Herein, a Cathelicidin-DM-derived peptide named Cath-DM-NT was identified from the skin of D. melanostictus. Previous studies have shown that Cathelicidin-DM has significant antibacterial activity, while Cath-DM-NT has no antibacterial activity. In this study, Cath-DM-NT is found to have lectin-like activity which can agglutinate erythrocytes and bacteria, and bind to lipopolysaccharide (LPS). In addition, Cath-DM-NT has antioxidant activity, which can scavenge 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and nitric oxide (NO) radicals and reduce Fe3+. Consistently, Cath-DM-NT can protect PC12 cells from H2O2-induced oxidative damage and carrageenan-induced paw edema, reduce malondialdehyde (MDA) and reactive oxygen species (ROS) accumulation, and restore superoxide dismutase (SOD) and glutathione (GSH) levels. Our study suggests that Cath-DM-NT can serve as a lead compound for the development of drugs with dual lectin and antioxidant effects.
科研通智能强力驱动
Strongly Powered by AbleSci AI