Biocatalytic Introduction of Chiral Hydroxy Groups using Oxygenases and Hydratases

Asymmetric introduction of hydroxy group is an important reaction in the industrial synthesis of valuable chiral compounds such as pharmaceuticals and fine chemicals. In this chapter, applications of monooxygenation enzymes, like cytochrome P450 monooxygenases and Fe(II)/α-ketoglutarate (α-KG)-dependent dioxygenases, are discussed. The use of a novel hydratase for introduction of hydroxy groups into unsaturated fatty acid is also described. These biocatalytic introductions of hydroxy groups using oxygenases and hydratases have advantages in regio- and stereoselective synthesis of chiral compounds.