Study on applicability of QAMS to Phellodendri Chinensis Cortex formula granules and cork pieces

OBJECTIVE To study feasibility and accuracy of quantitative analysis of multi-components by single marker(QAMS)for simultaneous determination of contents of five components in Phellodendri Chinensis Cortex formula granules and cork pieces.METHODS HPLC was used,with berberine as internal standard,relative correction factors(RCF)of phellodendrine,jatrorrhizine,pamatin and obakunone were calculated.According to RCF,contents of four components were simultaneously measured in Phellodendri Chinensis Cortex formula granules and cork pieces.Similarity was determined between measured values of QAMS method and real values of external standard method.RESULTS Within linear ranges,RCF values of phellodendrine,jatrorrhizine,pamatin and obakunone were0.235 0,0.890 7,0.739 8 and 0.769 8,respectively.RCF had a good reproducibility in various instruments and chromatographic columns(RSD=0.18%-0.63%).Results of QAMS method were validated by comparing with that of external standard method,and no obvious significant difference was found.CONCLUSION QAMS method can be used for quantitative analysis and quality evaluation of Phellodendri Chinensis Cortex formula granules and cork pieces.