Analysis of Tetracycline antibiotic residues in seafood by SPE-HPLC method

A method for simultaneous determination of oxytetracycline,tetracycline and chlortetracycline in seafood was successfully developed using high performance liquid chromatography.The mobile phase was composed of solium dihydrogen phosphate and acetonitrile.The detection wavelength was set at 356 nm and 371 nm.The results showed that three tetracycline antibiotics were well separated,and the limits of detection for three antibiotics were 14 ng for oxytetracycline,11 ng for tetracycline,and 34 ng for chlortetracycline.The recoveries of standard antibiotics were 12.61% for oxytetracycline,11.18% for tetracycline,and 28.10% for chlortetracycline.The relative standard deviation was 0.34%~2.79% for oxytetracycline,0.53%~3.36% for tetracycline,0.46%~5.45% for chlortetracycline.The method is rapid,sensitive,accurate and reliable,and can be used in the analysis of antibiotics residues in seafood.