In this experiment,we try to detect Streptococcus thermophilus in dairy products by serological way.Firstly,we prepared Streptococcus thermophilus serums by inoculating mice and rabbits.And then,using the serums detect Streptococcus thermophilus in dairy products by Enzyme linked Immunosorbent Assay(ELISA).We tried indirect ELISA and double antibody filling ELISA,finding both the ways can detect Streptococcus thermophilus in dairy products,and the minimum value of Streptococcus thermophiluss concentration which is capable of being determinated is about 107 mL-1.The result provide a base for serological rapid detecting of Streptococcus thermophilus in dairy products.