The Preparation of Edible Mussel Polysaccharide and It’s Anti-Tumor Activity In Vitro

Objective To prepare the polysaccharide from edible mussel and to investigate it’s in vitro anti-tumor activity.Methods The crude polysaccharide was extracted from edible mussel with hot water and the protein was removed using Sevage-enzymic method.The polysaccharide was purified using DEAE-Sepharose and Sepharose column chromatography.The in vitro anti-tumor activity of edible mussel polysaccharide was assessed using MTT method.Results The yield of edible mussel polysaccharide was 5.48%.The edible mussel polysaccharide(at 50 mg/L,100 mg/L,200 mg/L) had different degrees of inhibitory effects on HL-60 tumor cells in vitro(P0.05).Conclusion The polysaccharide prepared from edible mussel has inhibitory effect on HL-60 tumor cells in vitro.