A novel electrochemical biosensor for bisphenol A detection based on engineered Escherichia coli cells with a surface-display of tyrosinase

Electrochemical enzyme biosensors based on various modified electrodes have been developed for bisphenol A detection. However, enzyme activity loss and high cost limit their application. In this study, an electrochemical biosensor was developed for bisphenol A detection by displaying tyrosinase on the surface of Escherichia coli cells, followed by adsorption onto a glassy-carbon electrode. The biosensor exhibited a linear relationship between the concentration range of 0.01–100 nM BPA (R2 = 0.9967) and the current peak. The detection limit was 0.01 nM, which is higher than that of other chemically modified tyrosinase biosensors. For the detection of bisphenol A in tea and juice samples, the fabricated biosensor presented respectable accuracy comparable to that of high-performance liquid chromatography. Furthermore, high recovery rates (97.78%−100.32%) were obtained. Therefore, this biosensor could be a promising and reliable analytical tool for the detection of bisphenol A.