Phenotypic Characterization Of Immune Cells Isolated From Adenoids And Tonsils Of Children With Adenoid And Tonsil Hypertrophy

S A T U R D A Y 49 Phenotypic Characterization Of Immune Cells Isolated From Adenoids And Tonsils Of Children With Adenoid And Tonsil Hypertrophy L. P. Titov, A. M. Dashkevich, V. L. Chekan, L. M. DuBuske; Research Institute for Epidemiology and Microbiology, Minsk, BELARUS, Republica Scientific-and-Practical Center for Epidemiology and Microbiology, Minsk, BELARUS, Immunology Research Institute of New England, Gardner, MA. RATIONALE: The composition and immunophenotype of the lymphocytes subsets isolated from adenoids and tonsils of children with adenotonsil pathology may provide insight into tonsil and adenoid disease. METHODS: Samples tissues of adenoids and tonsils were obtained from 15 children undergoing adeno-tonsillectomy due adenoid and tonsil hypertrophy. Adenoids and tonsils were minced and the tissue digested with collagenase IVand DNase I. Released cells were filtered through 100mm nylon cell strainer. Immunophenotypic analysis of mononuclear cells was performed using FACSCalibur cytometer. RESULTS: CD19+ B-lymphocytes were present as a major lymphoid cell fraction (median 60.69%; range 51.95 to 61.64%), while CD3+ Tlymphocytes averaged 30.8 (range 22.27 to 34.55) %. The majority of cells among CD3+ T-lymphocytes were CD4+ T-cells helpers. Correlation existed between intensity of expression of CD3+T-lymphocytes and CD19+ B-lymphocytes (R50.7, p<0.05). Myeloid DC were 0.3 (range 0.22 to 0.44) % of the total isolated lymphoid cells and plasmacytoid DC were 0.44 (range 0.21 to 0.66) %. CONCLUSIONS: CD19+ B-lymphocytes are a major component of the total lymphoid cells isolated from adenotonsil tissue in children with adenoid and tonsil hypertrophy. Relatively small numbers of DC may be involved in the pathogenesis of adenotonsil hypertrophy.