生物传感器
荧光
分子内力
绿色荧光蛋白
荧光蛋白
化学
生物物理学
分子间力
费斯特共振能量转移
生物化学
细胞生物学
生物
分子
基因
立体化学
物理
量子力学
有机化学
作者
Yidan Ding,Jiao Li,Jhon R Enterina,Yi Shen,Issan Zhang,Paul Tewson,Gary Mo,Jin Zhang,Anne Marie Quinn,Thomas E. Hughes,Dusica Maysinger,Spencer C. Alford,Yan Zhang,Robert E. Campbell
出处
期刊:Nature Methods
[Springer Nature]
日期:2015-01-26
卷期号:12 (3): 195-198
被引量:119
摘要
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.
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