PI3K/AKT/mTOR通路
子宫肌瘤
平滑肌瘤
蛋白激酶B
肌层
细胞生长
胰岛素样生长因子
癌症研究
生物
信号转导
生长因子
内分泌学
细胞生物学
内科学
医学
病理
子宫
受体
遗传学
作者
Cuicui Wang,Yuan Sun,Shengyi Cong,Feiyue Zhang
出处
期刊:Cells Tissues Organs
[S. Karger AG]
日期:2022-05-23
卷期号:212 (2): 194-202
被引量:3
摘要
The present research aimed to evaluate the expression of insulin-like growth factor-1 (IGF-1) in uterine leiomyoma, and explore its relationship with the occurrence and development of uterine leiomyoma and potential signal pathways. qRT-PCR and enzyme-linked immunosorbent assay were used for estimating the levels of IGF-1 in human uterine leiomyoma compared to myometrium. The expression of cell proliferation and PI3K/AKT/mTOR signaling pathway-related proteins in uterine leiomyoma cells was evaluated by western blot. Cell viability analysis was performed by CCK-8 assay. Lentivirus infection was used for IGF-1 overexpression and knockdown in uterine leiomyoma cells. The IGF-1 expression level was elevated in human uterine leiomyomas compared to myometrium. IGF-1 promoted the cell viability of human uterine leiomyoma cells. Overexpression of IGF-1 induced the expression of pro-proliferation markers including c-Myc, PCNA, and cyclin D1 in uterine leiomyoma cells. IGF-1 elevated the phosphorylation levels of PI3K, AKT, and mTOR, thus modifying PI3K/AKT/mTOR signaling in uterine leiomyoma cells. IGF-1 promotes proliferation of human uterine leiomyoma cells via PI3K/AKT/mTOR pathway.
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