核酸酶
效应器
生物
DNA
核糖核酸
阿尔戈瑙特
核酸
细胞生物学
人口
遗传学
基因
RNA干扰
人口学
社会学
作者
Maria A Prostova,Anna Kanevskaya,Vladimir Panteleev,L. A. Lisitskaya,Kristina V. Tugaeva,Nikolai N. Sluchanko,Daria Esyunina,Andrey Kulbachinskiy
出处
期刊:Nature microbiology
日期:2024-04-15
标识
DOI:10.1038/s41564-024-01654-5
摘要
Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR–Cas, detect and cleave invader nucleic acids using complementary guides and the nuclease activities of pAgo or Cas proteins. However, not all pAgos are active nucleases. A large clade of short pAgos bind nucleic acid guides but lack nuclease activity, suggesting a different mechanism of action. Here we investigate short pAgos associated with a putative effector nuclease, NbaAgo from Novosphingopyxis baekryungensis and CmeAgo from Cupriavidus metallidurans. We show that these pAgos form a heterodimeric complex with co-encoded effector nucleases (short prokaryotic Argonaute, DNase and RNase associated (SPARDA)). RNA-guided target DNA recognition unleashes the nuclease activity of SPARDA leading to indiscriminate collateral cleavage of DNA and RNA. Activation of SPARDA by plasmids or phages results in degradation of cellular DNA and cell death or dormancy, conferring target-specific population protection and expanding the range of known prokaryotic immune systems. DNA recognition by Argonaute proteins activates effector nucleases that trigger indiscriminate DNA and RNA cleavage and induce abortive infection.
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