检出限
生物传感器
肺癌
适体
材料科学
微泡
分析物
纳米技术
癌症生物标志物
癌症研究
癌症
化学
小RNA
生物
分子生物学
色谱法
医学
生物化学
肿瘤科
内科学
基因
作者
J. Liu,Z. Liu,Chunqin Zhao,Yang Jiao,B. Li,Jiaju Shi,Zichao Chen,Zhen Zhang
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2024-01-01
摘要
Exosomal programmed death ligand-1 (ExoPD-L1) is a vital marker of immune activation in the early stages of tumor therapy and it can inhibit anti-tumor immune responses. However, due to the low expression of ExoPD-L1 in cancer cells, it is difficult to perform highly sensitive assays and accurately differentiate cancer sources. Therefore, we constructed a coaxial dual-path electrochemical biosensor for highly accurate identification and detection of ExoPD-L1 from lung cancer based on chemical-biological coaxial nanomaterials and nucleic acid molecular signal amplification strategies. The measurements showed that the detected ExoPD-L1 concentrations ranged from 6 × 102 particles per mL to 6 × 108 particles per mL, and the detection limit was 310 particles per mL. Compared to other sensors, the electrochemical biosensor designed in this study has a lower detection limit and a wider detection range. Furthermore, we also successfully identified lung cancer-derived ExoPD-L1 by analyzing multiple protein biomarkers expressed on exosomes through the "AND" logic strategy. This sensor platform is expected to realize highly sensitive detection and accurate analysis of multiple sources of ExoPD-L1 and provide ideas for the clinical detection of ExoPD-L1.
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