化学
适体
卟啉
配体(生物化学)
光动力疗法
循环肿瘤细胞
生物素化
硼酸
光敏剂
荧光
生物物理学
癌细胞
组合化学
光化学
癌症
生物化学
分子生物学
内科学
受体
有机化学
物理
生物
转移
医学
量子力学
作者
Qilong Hao,Taihe Han,Shuicheng Yan,Yi Fan,Xiaofeng Chen,Yan‐Ping Shi,Haixia Zhang
标识
DOI:10.1021/acs.analchem.5c03590
摘要
Circulating tumor cells (CTCs), as important markers for liquid biopsy, are of great significance for the early diagnosis of cancer and evaluation of postoperative efficacy. Currently, it is a challenge to capture rare CTCs in the complex environment of peripheral blood. In this study, a multifunctional dual-ligand MOF (MD-MOF) was prepared with porphyrin as the first ligand and a boronic acid group as the second ligand. In the MD-MOF, the porphyrin ligand can image the captured CTCs using its own fluorescence properties and further act as a photosensitizer to generate cytotoxic reactive oxygen species under light irradiation, killing the captured CTCs. The boronic acid group used to bond to the glycosylation site of avidin and self-assembled with the biotinylated aptamer to alleviate the inactivation of aptamers caused by interchain entanglement. MD-MOF can specifically capture MCF-7 cells with a capture efficiency as high as 93%. Even at an extremely low cell concentration (10 cells mL-1), the capture efficiency can still reach 76%. The captured cells can be eliminated within 5 min by laser irradiation or can be released without damage through a fructose competitive reaction. The multifunctional material integrated CTC capture, imaging, and photodynamic therapy, exploring the application potential in CTC capture.
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