多糖
化学
乙醇沉淀
糖醛酸
葱
食品科学
果糖
乙醇
生物化学
植物
生物
作者
Jun Yan,Chun Wang,Yun-Bo Yu,Liqing Wu,Tingting Chen,Ziwei Wang
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-03-01
卷期号:339: 128081-128081
被引量:56
标识
DOI:10.1016/j.foodchem.2020.128081
摘要
In the present study, three-phase partitioning (TPP) coupled with gradient ethanol precipitation (GEP) was developed for the first time to extract and isolate polysaccharides (GPSs) from raw garlic (Allium sativum L.) bulbs. Four kinds of fructose polymers, namely, GPS35, GPS50, GPS65, and GPS80, were obtained at the final ethanol precipitation concentrations of 35%, 50%, 65%, and 80% (v/v), respectively, and their physicochemical characteristics and in vitro biological activities were investigated. Results indicated that GPS80 had higher carbohydrate (86.68% ± 0.90%) and uronic acid (12.89% ± 0.09%) contents, lower weight-average molecular weight (8.93 × 103 Da), and looser surface morphology than the other three GPSs. Furthermore, among the four GPSs, GPS80 exhibited the strongest antioxidant capacities, inhibitory effects on α-amylase and α-glycosidase, and nitric oxide stimulatory activity on RAW264.7 macrophage cells in vitro. Therefore, this study provides a simple and feasible technological strategy for producing bioactive polysaccharides from raw Allium vegetables.
科研通智能强力驱动
Strongly Powered by AbleSci AI