聚苯胺
分子印迹
共聚物
材料科学
单体
化学工程
分子印迹聚合物
导电聚合物
两亲性
聚苯胺纳米纤维
检出限
水溶液
聚合物
选择性
聚合
化学
有机化学
色谱法
催化作用
工程类
复合材料
作者
Jing Luo,Jing Huang,Yunan Wu,Jun Sun,Wei Wei,Xiaoya Liu
标识
DOI:10.1016/j.bios.2017.02.035
摘要
In this work, a novel kind of water-dispersible molecular imprinted conductive polyaniline particles was prepared through a facile and efficient macromolecular co-assembly of polyaniline with amphiphilic copolymer, and applied as the molecular recognition element to construct protein electrochemical sensor. In our strategy, an amphiphilic copolymer P(AMPS-co-St) was first synthesized using 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and styrene (St) as monomer, which could co-assemble with PANI in aqueous solution to generate PANI particles driven by the electrostatic interaction. During this process, ovalbumin (OVA) as template protein was added and trapped into the PANI NPs particles owing to their interactions, resulting in the formation of molecular imprinted polyaniline (MIP-PANI) particles. When utilizing the MIP-PANI particles as recognition element, the resultant imprinted PANI sensor not only exhibited good selectivity toward template protein (the imprinting factor α is 5.31), but also a wide linear range over OVA concentration from 10–11 to 10−6 mg mL−1 with a significantly lower detection limit of 10–12 mg mL−1, which outperformed most of reported OVA detecting methods. In addition, an ultrafast response time of less than 3 min has also been demonstrated. The superior performance is ascribed to the water compatibility, large specific surface area of PANI particles and the electrical conductivity of PANI which provides a direct path for the conduction of electrons from the imprinting sites to the electrode surface. The outstanding sensing performance combined with its facile, quick, green preparation procedure as well as low production cost makes the MIP-PANI particles attractive in specific protein recognition and sensing.
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