果胶酸裂解酶
基因沉默
成熟
果胶
基因
RNA干扰
生物
软化
裂解酶
糖
基因表达
生物化学
植物
酶
果胶酶
核糖核酸
统计
数学
作者
Haozhan Ren,Qi Zhao,Youwei Feng,Peipei Tang,Yong Wang,Jun Jiang,Chunhua Hu,Yunxing Wang,Baolu Cui,Xiaodong Xie,Yan Li,Xue‐Jie Zhao,Huihui Gu,Jinyong Huang,Yanjie Zhang
标识
DOI:10.1016/j.postharvbio.2023.112368
摘要
Pectate lyase plays an important role in fruit development and ripening. Although earlier studies showed SlPL, a pectate lyase encoding gene, functions dominantly in tomato fruit softening, the functions of many other pectate lyase encoding genes involved in tomato fruit development and softening remain unknown. Here, gene-specific silencing of SlPL16 (Solyc06g083580) by RNA interference (RNAi) resulted in reduced pectate lyase enzyme activity, pericarp thickness, and soluble sugar content, and increased both the firmness and shelf life of tomato fruit. Through microscopic observation, it was found that the SlPL16-RNAi pericarp had reduced cell size, in comparison to the wild-type (WT). Moreover, gene silencing of SlPL16 resulted in the down-regulation of genes involved in cell wall degradation and pectin synthesis at various fruit developmental stages. Together, these results indicate that SlPL16 provides an important supplement of pectate lyase activity in tomato fruit softening, along with a fundamental role in cell expansion during early fruit development. Additionally, this study provides a potential gene locus in genetic engineering to improve fruit storage tolerance.
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