Insights to Fe(II) on the fate of humic acid and humic acid Fe complex with biogeobattery effect in simultaneous partial nitritation, anammox and denitrification (SNAD) system

The role of Fe(II) on the humic acid (HA) transformation and the effects of humic acid Fe (HA-Fe) on simultaneous partial nitrification, anammox and denitrification (SNAD) system were investigated. After adding Fe(II), the HA content decreased and the HA inhibition on the SNAD system was released. Results showed that Fe(II) and HA formed the lower water-soluble HA-Fe, promoting the HA removal. HA-Fe with stronger electron transfer capacity constituted the interface with microorganisms to forming the biogeobattery effect. This accelerated the microbial electron transfer, as well as improved the key enzymes and ATP, indicating that HA-Fe stimulated the microbial activity of the SNAD system. Microbial community and quorum sensing analysis further demonstrated that HA-Fe enhanced the mutual symbiosis between electroactive and nitrogen removal bacteria, to ensure the stability of the SNAD system. The study provided references for efficient HA removal and revealed the biogeobattery effect of HA-Fe in the SNAD system.