Limibacterium fermenti gen. nov., sp. nov., a novel member of the family Dysgonomonadaceae isolated from the Chinese strong aroma-type Baijiu fermentation system

An anaerobic isolate, designated me31^T, was isolated from pit mud in Yibin, Sichuan Province, PR China. Phylogenetic results based on 16S rRNA gene sequence showed that strain me31^T belongs to the family Dysgonomonadaceae, and the most closely related isolated relatives were Seramator thermalis SYSU GA16112^T (93.65%) and Proteiniphilum propionicum JNU-WLY501^T (93.22%). The DNA G+C content was 44.26 mol%. The ANI and AAI values between strain me31^T and the closely related strains were 69.25-71.18% and 69.59-71.15%, respectively. Cells of strain me31^T were Gram-stain-negative, rod-shaped and non-motile. Growth of strain me31^T was observed at 25-37 °C, pH 6.0-8.0 and a salt tolerance range of 0-1.0% (w/v). The predominant respiratory quinone was MK-9. The major fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and C_{17 : 0} 2OH. The polar lipids of strain me31^T were found to consist of phosphatidylethanolamine, three unidentified phospholipids, three unidentified phosphoglycolipids, one unidentified phosphoglycolipid, one unidentified lipid, two unidentified glycolipids and one aminophosphoglycolipid. According to the results of morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic analysis, strain me31^T represents a novel species of a novel genus of the family Dysgonomonadaceae, for which the name Limibacterium fermenti gen. nov., sp. nov. is proposed. The type strain is me31^T (=GDMCC 1.4237^T=KCTC 25756^T=WMCC 10035^T).