Single-atom nanozymes for enhanced electrochemical biosensing: A review

Enzyme-based electrochemical biosensors have broad and significant applications in biomedical, environmental monitoring, and food safety fields. However, the application of natural enzymes is limited due to issues such as poor stability, complex preparation, and high cost. Single-atom nanozymes (SAzymes), with their unique catalytic properties and efficient enzyme-like activities, present a promising alternative in the field of electrochemical biosensing. Compared to traditional enzymes, SAzyme offer enhanced stability and controllability, making them particularly effective in complex detection environments. This work presents the first systematic review of the progress made since 2018 in the use of SAzymes as alternatives to natural enzymes in electrochemical biosensors, and presents the latest advancements in this area. The review begins with a discussion of various enzyme-like activities of single-atom materials, including peroxidase (POD)-like, oxidase (OXD)-like, catalase (CAT)-like, and superoxide dismutase (SOD)-like activities. It then explores the advantages of SAzymes in improving the performance of electrochemical biosensors from multiple perspectives. The review also summarizes the applications of SAzyme-based electrochemical sensors for reactive oxygen species (ROS), metabolites, neurotransmitters , and other analytes, highlighting specific examples to elucidate underlying catalytic mechanisms and understand fundamental structure-performance relationships. In the final section, the challenges faced by SAzyme-based electrochemical biosensing are discussed, along with potential solutions. • First review on SAzymes as alternatives to enzymes in electrochemical sensors. • Discussion of SAzymes mimicking POD, OXD, CAT, and SOD-like activities. • Clarifying catalytic mechanisms and structure-performance relationships. • Applications of SAzymes in electrochemical biosensors since 2018. • Challenges in SAzyme-based electrochemical biosensing and proposed solutions.