粘合连接
细胞生物学
紧密连接
连环素
细胞结
肌动蛋白
顶端细胞
生物
连接蛋白
化学
细胞粘附
上皮
遗传学
钙粘蛋白
生物化学
Wnt信号通路
信号转导
细胞
作者
Pierre Mangeol,Dominique Massey-Harroche,Michaël Sebbagh,Fabrice Richard,André Le Bivic,Pierre-François Lenne
标识
DOI:10.1073/pnas.2316722121
摘要
Cell–cell apical junctions of epithelia consist of multiprotein complexes that organize as belts regulating cell–cell adhesion, permeability, and mechanical tension: the tight junction ( zonula occludens ), the zonula adherens ( ZA ), and the macula adherens . The prevailing dogma is that at the ZA , E-cadherin and catenins are lined with F-actin bundles that support and transmit mechanical tension between cells. Using super-resolution microscopy on human intestinal biopsies and Caco-2 cells, we show that two distinct multiprotein belts are basal of the tight junctions as the intestinal epithelia mature. The most apical is populated with nectins/afadin and lined with F-actin; the second is populated with E-cad/catenins. We name this dual-belt architecture the zonula adherens matura . We find that the apical contraction apparatus and the dual-belt organization rely on afadin expression. Our study provides a revised description of epithelial cell–cell junctions and identifies a module regulating the mechanics of epithelia.
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