生物反应器
混合(物理)
桨
放大
同种类的
生物技术
比例(比率)
生物
生物系统
工艺工程
生化工程
计算机科学
物理
植物
工程类
热力学
经典力学
量子力学
操作系统
作者
Masaaki Ueki,Tadashi Suzuki,Yoshikazu Kato
标识
DOI:10.1016/j.jbiosc.2023.12.008
摘要
A substantial number of human iPS cells (hiPSCs) is needed for cell therapy to be successful against various diseases. We previously reported on a bioreactor with reciprocal mixing that produces specific physical properties that differ from those of conventional bioreactors with rotary paddle stirring. Moreover, such reactors not only provide a homogeneous environment but also allow the control of spheroid size by changing the mixing speed. In this study, we applied this bioreactor to the large-scale cultivation of hiPSCs. Approximately 10 billion hiPSCs were obtained from 2.0 L of culture, and the high expression of pluripotency markers was maintained. Our findings indicate that a bioreactor with reciprocal mixing can be used for large-scale hiPSC cultivation.
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