神经干细胞
神经发生
神经保护
神经母细胞
移植
细胞生物学
生物
细胞分化
转录因子
干细胞
药理学
生物化学
医学
基因
内科学
作者
Yuxiao Ma,Zheng-Hui He,Jiangchang Wang,Ping Zheng,Zixuan Ma,Qian Liang,Zhihua Qiao,Xiongfei Zhao,Jialin Huang,Weiji Weng,Jiyao Jiang,Junfeng Feng
出处
期刊:iScience
[Elsevier]
日期:2024-03-01
卷期号:: 109435-109435
标识
DOI:10.1016/j.isci.2024.109435
摘要
Both therapeutic hypothermia and neural stem cells (NSCs) transplantation have shown promise in neuroprotection and neural repair after brain injury. However, the effects of therapeutic hypothermia on neuronal differentiation of NSCs are not elucidated. In this study, we aimed to investigate whether mild hypothermia promoted neuronal differentiation in cultured and transplanted human NSCs (hNSCs). A significant increase in neuronal differentiation rate of hNSCs was found when exposed to 35°C, from 33% to 45% in vitro and from 7% to 15% in vivo. Additionally, single-cell RNA sequencing identified upregulation of RNA-binding motif protein 3 (RBM3) in neuroblast at 35°C, which stabilized the SRY-box transcription factor 11 (SOX11) mRNA and increased its protein expression, leading to an increase in neuronal differentiation of hNSCs. In conclusion, our study highlights that mild hypothermia at 35°C enhances hNSCs-induced neurogenesis through the novel RBM3-SOX11 signaling pathway, and provides a potential treatment strategy in brain disorders.
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