急性肾损伤
中性粒细胞弹性蛋白酶
医学
弹性蛋白酶
中性粒细胞胞外陷阱
病理生理学
肺
粒细胞生成
免疫学
炎症
内科学
酶
生物
祖细胞
生物化学
遗传学
干细胞
作者
Weiqiang Jing,Fei Qin,Xing Guo,Yanlin Sun,Can Yan,Changjian Qiu,Masato Tanaka,Benkang Shi,Yunxue Zhao
标识
DOI:10.1016/j.intimp.2018.07.032
摘要
Acute lung injury (ALI) is a serious complication among patients with acute kidney injury (AKI) that is a systemic inflammatory disease with high morbidity and mortality. The pathophysiology of AKI-associated ALI is poorly understood. G-CSF regulates the production and function of neutrophils that mediate lung injury via elastase and other mediators. Here, we used a mouse model of adenine-induced AKI to determine the roles of G-CSF and neutrophil elastase in AKI-associated ALI. We confirmed that ALI was associated with high serum G-CSF levels, and elevated neutrophil elastase activity in the lungs and serum of mice with adenine-induced AKI. Systemic administration of G-CSF-specific neutralizing antibody normalized granulopoiesis, pulmonary neutrophil infiltration, and neutrophil elastase activity, conferring improved lung architecture in mice with adenine-induced AKI. Further studies revealed that macrophages secreted G-CSF upon urea stimulation. Consequently, G-CSF could be a target for new anti-lung injury strategy in patients with AKI.
科研通智能强力驱动
Strongly Powered by AbleSci AI