分析物
检出限
生物分子
生物标志物
化学
静电学
灵敏度(控制系统)
表征(材料科学)
纳米技术
制作
色谱法
静电
周转时间
分析化学(期刊)
材料科学
计算机科学
物理
生物化学
物理化学
工程类
病理
电子工程
操作系统
医学
替代医学
量子力学
作者
Yifan Dai,Liang Yuan Chiu,Yinuo Chen,Shiyi Qin,Xiaowei Wu,Chung Chiun Liu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2018-12-24
卷期号:4 (1): 161-169
被引量:26
标识
DOI:10.1021/acssensors.8b01126
摘要
A noninvasive, highly sensitive universal immunosensor platform for protein-based biomarker detection is described in this Article. A neutral charged sensing environment is constructed by an antibody with an oppositely charged amino acid as surface charge neutralizer. By adjusting the pH condition of the testing environment, this neutral charged immunosensor (NCI) directly utilizes the electrostatic charges of the analyte for quantification of circulating protein markers, achieving a wide dynamic range covering through the whole picomole level. Comparing with previous studies on electrostatic charges characterization, this NCI demonstrates its capability to analyze not only the negatively charged biomolecules but also positively charged analytes. We applied this NCI for the detection of HE4 antigen with a detection limit at 2.5 pM and Tau antigen with a detection limit at 0.968 pM, demonstrating the high-sensitivity property of this platform. Furthermore, this NCI possesses a simple fabrication method (less than 2 h) and a short testing turnaround time (less than 30 min), providing an excellent potential for further clinical point-of-care applications.
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