衣霉素
塔普斯加尔金
内质网
糖基化
未折叠蛋白反应
活力测定
细胞毒性
细胞凋亡
化学
下调和上调
糖基化终产物
细胞生物学
药理学
医学
内分泌学
内科学
生物化学
生物
糖尿病
体外
基因
作者
Bin Huang,Huangqin Chen
出处
期刊:DEStech Transactions on Engineering and Technology Research
[DEStech Publications]
日期:2019-01-24
卷期号: (ecae)
被引量:1
标识
DOI:10.12783/dtetr/ecae2018/27755
摘要
Purposes: Increased advanced glycation end products (AGEs) plays critical role in the exacerbation of periodontitis in diabetic patients. Though our previous findings confirmed the importance of endoplasmic reticulum stress (ERS) in diabetic-relative periodontitis, the regulating action of ERS on AGEs-induced apoptosis remains uncertain. Methods: human periodontal ligament cells (hPDLCs) were pretreated with ERS inducer tunicamycin or thapsigargin, followed by AGEs. Cell viability and expression ofGRP78 were analyzed. Results: AGEs decreased cell viability. Tunicamycin or thapsigargin pretreatment inhibited AGEs-induced cytotoxicity and increased the expression of GRP78. Knockdown of GRP78 significantly blunts the protective effect of tunicamycin or thapsigargin. Conclusion: These data suggest that tunicamycin or thapsigargin suppresses AGEs-induced cytotoxicity by upregulation of GRP78.
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