重组工程
生物
脓肿分枝杆菌
转化(遗传学)
质粒
基因组DNA
核酸
DNA
突变
遗传学
生物安全
计算生物学
细菌
突变
分枝杆菌
生物技术
基因
作者
Halima Medjahed,Anil Kumar Singh
标识
DOI:10.1002/9780471729259.mc10d02s18
摘要
This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.
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