超氧化物
食腐动物
化学
生物化学
自由基清除剂
发光测量
激进的
抗氧化剂
酶
生物物理学
生物
发光
材料科学
光电子学
作者
Livia Saleh,Christoph Plieth
出处
期刊:Nature Protocols
[Springer Nature]
日期:2010-09-16
卷期号:5 (10): 1635-1641
被引量:18
标识
DOI:10.1038/nprot.2010.121
摘要
In all living cells, levels of reactive oxygen species are kept in check by antioxidative activities. Superoxide radicals are dismutated by superoxide dismutases, by other enzymes and by nonenzymatic compounds. This protocol describes the quantification of superoxide scavenging activities (SOSA). It is based on the inhibition of chemiluminescence emitted by coelenterazine when oxidized by superoxide. SOSA is a summary parameter comprising all high-molecular-weight superoxide scavengers in a biological sample. Enzymes and nonenzymatic scavengers can also be distinguished. The SOSA assay is quick, reproducible and applicable to fields as diverse as medical diagnostics, food sciences, or agriculture. The protocol presented here requires about 2 working days to complete.
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