Studies on Haptoglobin. I

The specific antibody to human adult hemoglobin A1 was prepared. And the immunochemical properties of haptoglobin which also binds specifically with hemoglobin were compared with Fig. 3. Complement fixation reaction between Hb Al and (a) antihemoglobin antibody or (b) human Hp. 4: 100% hemolysis, 3: 75% hemolysis, 2: 50% hemolysis, 1: 25% hemolysis, 0: non-hemolysis. those of antihemoglobin A1 antibody. Hemoglobin-haptoglobin shows neither precipitation reaction nor complement fixation reaction under conditions in which hemoglobin-antihemoglobin antibody shows these immunological reactions.