生物修复
六价铬
寡养单胞菌
铬
X射线光电子能谱
核化学
化学
细菌
扫描电子显微镜
环境化学
16S核糖体RNA
材料科学
生物
化学工程
有机化学
工程类
复合材料
遗传学
作者
Lin Li,Xiufang Shang,Xiaojie Sun,Xinfeng Xiao,Junmin Xue,Yu Gao,Haijun Gao
标识
DOI:10.1016/j.eti.2021.101409
摘要
A gram-negative bacterial strain capable of resisting hexavalent chromium was isolated and evaluated for its characteristics. The strain was designated as Stenotrophomonas acidaminiphila 4-1 according to the 16S rDNA sequence and phylogenic analysis. It was founded that sufficient nutrients were more suitable for intracellular enrichment of chromium, such as LB medium. Extracellular polymeric substances were observed as binding sites for chromium entering bacterial cells by transmission electron microscopy (TEM) and scanning transmission electron microscope-energy dispersive spectroscopy (STEM-EDS) analysis. Two sharp peaks at 577.18 eV and 578.24 eV were assigned to Cr 2p3/2 in X-ray photoelectron spectroscopy (XPS) spectra, illustrating only Cr(III) and Cr(VI) forms existed in stain 4-1. Possible removal mechanisms of chromium were also described. The removal rate of Cr(VI) was 75.69% with initial substrate concentration of 15 mg L −1 after 7d incubation. The microbial biomasses which cultured below Cr(VI) of 100 mg L −1 were varied slightly. And strain 4-1 could tolerate high concentration of Cr(VI) up to 1000 mg L −1. Removal rate of Cr(VI) was not obviously affected with diesel. The results showed that Stenotrophomonas acidaminiphila 4-1 is an excellent bacterial candidate for the bioremediation of chromium or combined with diesel contaminated sites.
科研通智能强力驱动
Strongly Powered by AbleSci AI