Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment

A heterotrophic nitrifying-aerobic denitrifying bacterium isolated from deep-sea sediment was identified as Pseudomonas bauzanensis DN13-1. Nitrogen (N) removal capability and relative expression of nitrification and denitrification genes of this strain were investigated. The NO2−-N, NO3−-N and NH4+-N removal efficiencies were 98.82%, 65.87% and 98.89%, respectively, and strain DN13-1 could efficiently remove mixed N. Meanwhile, other inorganic N was not accumulated during these N removal processes. Genomic analysis indicated that genes nirS, norB, nosZ, nasA and putative amo were identified. The relative expression of functional genes by real-time PCR (qPCR) further confirmed nitrite, nitrate and ammonium removal pathways of strain DN13-1 under aerobic condition. Especially, the ammonium removal pathway of this strain was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification directly. Taken together, strain DN13-1 possesses particularity to efficiently remove N, which guarantees its promising application in aquaculture wastewater treatment.