Evaluation of the effects of two techniques for analysis the formation mechanism in a rhesus D- index case

Objective To evaluate the effects of serological typing and PCR-SSP genotyping which were used for Rh blood typing and genetic basis of the Rh D- index case and her family members. Methods Rh D,C, c, E, e antigens were detected by serological typing. The characteristic sequence fragments of RHD and RHCE were amplified by PCR-SSP with a commercial reagent. Results Rh phenotypes of the proband' s family members were normal. The genotypes of PCR were consistent with their serological phenotypes. The full specific RHD bands of the proband were found. No specific RHCE bands were detected. The full specific bands of RHD and RHCE of her family members were normal. Conclusion Serological typing and PCR-SSP genotyping were complementary to RhD- diagnosis. Combination of the two technologies can help to analyze the genetic background of RhD-. Key words: RhD-; Serological typing; PCR-SSP; Genotyping; Genetic background