NMDA scRNAseq

PBMCs were obtained from 16 French patients with anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis and 16 French matched controls. Single cell 3’ and 5’ libraries were prepared by Chromium Single Cell 3’ Reagent Kits (v3.1 Chemistry) and Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 with Feature Barcode technology for Cell Surface Protein, respectively. Sequencing data were processed using the 10x Genomix Cell Ranger v6.0. Singlet was identified using demuxlet.