可重用性
炸薯条
检出限
量子点
免疫分析
微流控
聚二甲基硅氧烷
生物传感器
色谱法
化学
信号(编程语言)
材料科学
纳米技术
生物系统
光电子学
分析化学(期刊)
计算机科学
生物
抗体
电信
程序设计语言
免疫学
软件
作者
Xinran Xiang,Qinghua Ye,Yuting Shang,Fan Li,Baoqing Zhou,Yanna Shao,Chu-Fang Wang,Jumei Zhang,Liang Xue,Moutong Chen,Yu Ding,Qi Wu
标识
DOI:10.1016/j.bios.2021.113394
摘要
To achieve rapid and sensitive detection of aflatoxin B1 (AFB1), we developed a polydimethylsiloxane gravity-driven cyclic microfluidic chip using the two-signal mode strategy. The structural design of the chip, together with the two-wavelength quantum dot ratio fluorescence, effectively eliminates the influence of environmental factors, improves the signal stability, and ensures that the final detection result positively correlates with the target concentration. Moreover, the theoretical analysis performed for the established physical model of the three-dimensional reaction interface inside the chip confirmed the improved reaction rate of immune adsorption in the microfluidic strategy. Overall, the method exhibited a wide analytic range (0.2–500 ng mL−1), low detection limit (0.06 ng mL−1), high specificity, good precision (coefficient of variation < 5%), excellent reusability (20 times, 89.1%) and satisfactory practical sample analysis capacity. Furthermore, the reusability and designability of this chip provide a reliable scheme for field detection of AFB1, analysis of other small molecules, and establishment of high-throughput detection systems under different conditions.
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