生物传感器
变构调节
纳米技术
计算机科学
蛋白质工程
分析物
化学
计算生物学
受体
生物
材料科学
生物化学
物理化学
酶
作者
C.J. Jackson,Alisha Anderson,Kirill Alexandrov
标识
DOI:10.1016/j.sbi.2022.102424
摘要
Protein biosensors play increasingly important roles in cell and neurobiology and have the potential to revolutionise the way clinical and industrial analytics are performed. The gradual transition from multicomponent biosensors to fully integrated single chain allosteric biosensors has brought the field closer to commercial applications. We evaluate various approaches for converting constitutively active protein reporter domains into analyte operated switches. We discuss the paucity of the natural receptors that undergo conformational changes sufficiently large to control the activity of allosteric reporter domains. This problem can be overcome by constructing artificial versions of such receptors. The design path to such receptors involves the construction of Chemically Induced Dimerisation systems (CIDs) that can be configured to operate single and two-component biosensors.
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