Nowadays the use of molecular tool is gaining importance for the proper and precise identification of any biological organisms. The technique is successfully adopted for the precise identification of Bacillus spp. The available literature on identification of Bacillus spp. at molecular level were collected and presented below. The review will be useful to the researchers working on this line as the identification of Bacillus spp. using morphological and cultural characters, biochemical tests etc. are time consuming, cumbersone and lacks precision.