To use the stem of Marsdenia officinalis Tsiang et P.T.Li as the test materials,to get MOSE by ethanol and water extract,to study its effect on the sperm activity in vitro of Kunming species male mice.It is proved that MOSE in vitro on male mice has the function of anti-fertility and its activity is of dependent relation with the dosage,which shows the stem of Marsdenia officinalis possesses the activity composition of anti-fertility and obvious functions.The composition is mainly ethanol solubility.